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qizhenzengmiankeli for pseudorabies using method

  • Transcriptome-wide survey of pseudorabies virus

    2018-6-19 · Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very …

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  • Differential diagnosis of PRV-infected versus

    A novel time-resolved fluorescence blocking lateral flow immunoassay (TRF-BLFIA) was developed for on-site differential diagnosis of pseudorabies virus (PRV)-infected and vaccinated pigs using europium nanoparticles (EuNPs)-labeled virion antigens and high titer PRV gE monoclonal antibodies (PRV gE- …

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  • Loop-mediated isothermal amplification

    2008-7-1 · A rapid, convenient and reliable pseudorabies virus (PRV) detection system was developed by using the loop-mediated isothermal amplification (LAMP) method. Six special primers were designed successfully based on the PRV DNA-binding protein (DBP) gene. The assay was optimized to amplify PRV DNA by incubation at 63 °C for 1 h.

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  • CN101695573B - Method for producing pseudorabies

    The invention provides a method for producing pseudorabies living vaccines by using a subculture cell source and a pseudorabies living vaccine product thereof. The method comprises the following steps: culturing pseudorabies virus low-virulent strains by using subculture cells; harvesting the strains to obtain cell culture venom; and then adding a stabilizing agent and an antibiotic into the ...

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  • Immunoassay for antibodies to pseudorabies virus

    I claim: 1. The method for testing swine serum to determine the presence of antibodies to pseudorabies virus as distinguished from antibodies to lectin-binding pseudorabies glycoproteins in which an enzyme-linked immunosorbent assay is is performed on the swine serum using a pseudorabies virus antigen preparation, wherein the improvement comprises employing as said pseudorabies antigen ...

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  • CN103865886A - Culture method of porcine

    2019-11-1 · Pseudorabies virus (PRV) is a promising vaccine vector due to its distinctive features including many nonessential replication regions and a broad host range. Foreign genes of other viruses have been successfully inserted into and expressed in PRV and these recombinant viruses are very likely to induce humoral and/or cellular responses in immunized animals.

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  • CN101695572B - Method for producing pseudorabies ...

    The invention discloses a culture method of porcine pseudorabies virus; hamster kidney cells (BHK-21) are used as a cell source for culturing the porcine pseudorabies virus, and while cell passage, the virus is inoculated, so that multiplication culture of the virus is realized. The method has the characteristics of simple technology, high increment, high yield and low cost, and a vaccine ...

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  • CN102988974B - Method for preparing porcine

    The invention provides a method for producing a pseudorabies attenuated vaccine by using a bioreactor and a pseudorabies attenuated vaccine product. After being sterilized, the bioreactor and a micro carrier are inoculated with cells for producing the vaccine, and a cell growth medium is added for culture. A maintenance medium containing attenuated strains of pseudorabies viruses are ...

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  • Supplemental Assay Method for Pseudorabies Virus

    2014-12-30 · Supplemental Assay Method for Pseudorabies Virus Challenge Test in Swine UNCONTROLLED COPY 5.2.2 9 CFR 113.213 (c)(2)(vi): If at least 4 of the 5 CONT swine do not develop CNS signs or die, the test is inconclusive and may be repeated. For each

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  • An indirect double-antibody sandwich enzyme-linked ...

    An indirect double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) using baculovirus-expressed antigen for the detection of antibodies to glycoprotein E of pseudorabies virus and comparison of the method with blocking ELISAs

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  • Transcriptome-wide survey of pseudorabies virus

    Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very complex transcriptome. Here, we present a large RNA-Seq dataset, derived from both short- and …

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  • Different methods of real-time PCR for detection of ...

    Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR.

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  • Global stability of a pseudorabies virus model with ...

    2020-8-5 · Porcine pseudorabies infection is an acute infectious disease caused by pseudorabies virus. In this paper, we formulate a mathematical susceptible-incubating-infected-treated (SEIT) model with vertical transmission. The existence and stability of the equilibrium points of the model are characterized by the basic reproduction number Re_0.

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  • Construction of bovine herpesvirus-1 (BHV-1)

    2019-4-12 · We present a highly efficient viral genome manipulation method using CRISPR-guided cytidine deaminase. We cloned pseudorabies virus genome into bacterial artificial chromosome, and used CRISPR-guided cytidine deaminase to directly convert cytidine (C) to uridine (U) to induce premature stop mutagenesis in viral genes.

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  • RUNX1-mediated alphaherpesvirus-host trans-species ...

    We have improved the method for constructing recombinants of bovine herpesvirus type-1 (BHV-1). Using this method, we constructed three recombinants in which the pseudorabies virus (PRV) thymidine kinase (tk) gene was inserted at three different sites in the unique short region of BHV-1. These three sites are located in the open reading frame of gE, gG and gI genes.

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  • A new method for rapid screening of hybridoma cell

    Like most DNA viruses, herpesviruses precisely deliver their genomes into the sophisticatedly organized nuclei of the infected host cells to initiate subsequent transcription and replication. However, it remains elusive how the viral genome specifically interacts with the host genome and hijacks host transcription machinery. Using pseudorabies virus (PRV) as model virus, we performed ...

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  • Two Detailed Plaque Assay Protocols for the

    2021-6-8 · While using the ELISA-based method, it is necessary to conduct at least three subcloning cycles based on the limiting dilution method to obtain pure positive hybridoma clones after cell fusion (about 48 days), preparation and purification of ascites antibodies (about 20 days), antibody labeling and sandwich ELISA screening (about 2 days).

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  • Supplemental Assay Method for Pseudorabies Virus

    2014-12-30 · Supplemental Assay Method for Pseudorabies Virus Challenge Test in Swine UNCONTROLLED COPY 5.2.2 9 CFR 113.213 (c)(2)(vi): If at least 4 of the 5 CONT swine do not develop CNS signs or die, the test is inconclusive and may be repeated. For each

    Get Price
  • Supplemental Assay Method for Titration of Pseudorabies ...

    2015-2-20 · Supplemental Assay Method for Titration of Pseudorabies Virus Neutralizing Antibody (Constant Virus-Varying Serum Method) UNCONTROLLED COPY 1. Introduction This Supplemental Assay Method (SAM) describes an in vitro assay method which uses plaque reduction in a cell culture system to determine the antibody titer of serum against Pseudorabies ...

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  • Construction of recombinant pseudorabies viruses by

    We describe a simple and efficient method to obtain recombinant pseudorabies virus (PRV) in mammalian cells by using the PRV BACs, PBAC80 deficient in pac sequences and PBAC90 deficient in the IE180 gene. These essential viral sequences were …

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  • CULTIVATION OF PSEUDORABIES VIRUS

    attempted to cultivate pseudorabies virus by the method devised by Flexner and Noguchi (6) for the cultivation of the globoid bodies in poliomyelitis. The first series of Sangiorgi's cultures was still in- fectious after 8 days' incubation at 37°C. The first series of sub- ...

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  • The potency testing of pseudorabies vaccines in pigs.

    The statistical analysis of results optimizes the experimental parameters and gives rise to a quantitative test which discriminates between vaccinates and controls and substantiates a proposal for a minimum vaccine potency requirement. The implementation of the method proposed is compatible with current practice in control laboratories.

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  • Transcriptome-wide survey of pseudorabies virus

    Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very complex transcriptome. Here, we present a large RNA-Seq dataset, derived from both short- and long-read sequencing. The dataset contains 1.3 million 100 bp paired-end reads that were obtained from the Illumina random-primed libraries, as ...

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  • Mapping and sequence of the gene for the

    1985-4-1 · Walboomers JM, Schegget JT. A new method for the isolation of herpes simplex virus type 2 DNA. Virology. 1976 Oct 1; 74 (1):256–258. [Google Scholar] Wathen MW, Wathen LM. Isolation, characterization, and physical mapping of a pseudorabies virus mutant containing antigenically altered gp50. J Virol. 1984 Jul; 51 (1):57–62.

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  • Transcriptome-wide survey of pseudorabies virus

    2018-6-19 · Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very …

    Get Price
  • Detection of pseudorabies virus antibody in swine oral ...

    2020-10-13 · Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA . Abstract . We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs/group): negative

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  • Highly Efficient Base Editing in Viral Genome Based on ...

    2020-5-4 · viral genome manipulation method using CRISPR-guided cytidine deaminase. We cloned pseudorabies virus genome into bacterial artificial chromosome, and used CRISPR-guided cytidine deaminase to directly convert cytidine (C) to uridine (U) to induce premature stop mutagenesis in viral genes. The editing efficiencies were 100%.

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  • Supplemental Assay Method for Titration of Pseudorabies ...

    2015-2-20 · Supplemental Assay Method for Titration of Pseudorabies Virus Neutralizing Antibody (Constant Virus-Varying Serum Method) UNCONTROLLED COPY 1. Introduction This Supplemental Assay Method (SAM) describes an in vitro assay method which uses plaque reduction in a cell culture system to determine the antibody titer of serum against Pseudorabies ...

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  • Supplemental Assay Method for Titration of Pseudorabies ...

    2014-10-16 · Supplemental Assay Method for Titration of Pseudorabies Virus in Vaccines UNCONTROLLED COPY 5. Aseptically add: a. 10 mL L-glutamine (200 mM) b. 5 mL lactalbumin hydrolysate or edamine c. 50 µg/mL gentamicin sulfate 6. Store at 2°- 7°C. 2.2.4 Growth Medium 1. 900 mL of MEM 2. Aseptically add 100 mL gamma-irradiated fetal bovine serum (FBS). 3.

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  • Construction of recombinant pseudorabies viruses by

    We describe a simple and efficient method to obtain recombinant pseudorabies virus (PRV) in mammalian cells by using the PRV BACs, PBAC80 deficient in pac sequences and PBAC90 deficient in the IE180 gene. These essential viral sequences were …

    Get Price
  • CULTIVATION OF PSEUDORABIES VIRUS

    attempted to cultivate pseudorabies virus by the method devised by Flexner and Noguchi (6) for the cultivation of the globoid bodies in poliomyelitis. The first series of Sangiorgi's cultures was still in- fectious after 8 days' incubation at 37°C. The first series of sub- ...

    Get Price
  • Pseudorabies Virus: A Highly Specific Transneuronal Cell ...

    2003-9-23 · systematic use of the transneuronal viral labeling method for neuroanatomical studies. Since then, other groups have applied this method to study CNS pathways, and various neurotropic viruses have been used, including pseudorabies virus (Martin and …

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  • Transcriptome-wide survey of pseudorabies virus

    2018-6-19 · Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very …

    Get Price
  • Use of lambda gt11 to isolate genes for two

    1986-10-1 · DNA sequence of the gene for pseudorabies virus gp50, a glycoprotein without N-linked glycosylation. J Virol. 1986 Aug; 59 (2):216–223. [Europe PMC free article] [Google Scholar] Rea TJ, Timmins JG, Long GW, Post LE. Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells.

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  • Detection of pseudorabies virus antibody in swine oral ...

    2020-10-13 · Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA . Abstract . We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs/group): negative

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  • CRISPR/Cas9‐mediated 2‐sgRNA cleavage facilitates ...

    THE JOURNAL † RESEARCH † www.fasebj.org CRISPR/Cas9-mediated 2-sgRNA cleavage facilitates pseudorabies virus editing Yan-Dong Tang,1 Jin-Chao Guo,1 Tong-Yun Wang,1 Kuan Zhao, Ji-Ting Liu, Jia-Cong Gao, Zhi-Jun Tian, Tong-Qing An,2 and Xue-Hui Cai3 State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin,

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  • Pseudorabies Virus: A Highly Specific Transneuronal Cell ...

    2003-9-23 · systematic use of the transneuronal viral labeling method for neuroanatomical studies. Since then, other groups have applied this method to study CNS pathways, and various neurotropic viruses have been used, including pseudorabies virus (Martin and …

    Get Price
  • EP 2985288 A4 20160608 - PORCINE PSEUDORABIES

    452033698 - EP 2985288 A4 20160608 - PORCINE PSEUDORABIES VIRUS, VACCINE COMPOSITION AND PREPARATION METHOD AND USE THEREOF - [origin: EP2985288A1] Provided in the present invention is a vaccine composition comprising an immune amount of attenuated live vaccine, inactivated vaccine, subunit vaccine, synthetic vaccine, or genetically engineered vaccine of the porcine pseudorabies …

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  • Live attenuated pseudorabies virus developed using the ...

    The method is simple, requiring only the design of an effective single guide RNA (sgRNA) that is specific to a given target gene. DNA viruses can easily be edited, and the only delay involves determining the tar-geted sequence. In this study, we attempted to attenuate a current epidemic PRV variant strain using the CRISPR/Cas9 system. 2.

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  • Recombinant pseudorabies virus with gI/gE deletion ...

    2021-4-14 · Pseudorabies (PR), widely known as Aujeszky′s disease, is an infectious disease for most livestock and wild ani-mals caused by the pseudorabies virus (PRV). Pigs are a natural host while a variety of other vertebrates such as cattle, sheep, dogs, cats, goats, and rabbits, can be in-fected [1–4]. PRV infection in pigs produces different

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  • Long-term Cre-mediated retrograde tagging of neurons

    2020-1-15 · Since 2011, to control the spread of pseudorabies (PR), US7/US8/UL23-deleted recombinant PRV (rPRV) vaccines based on current variants have been developed. The vaccines can provide effective immune protection to pigs, but fur-bearing animals, such as dogs, foxes, and minks, are increasingly infected by PRV due to consuming contaminated raw meat or offal from immunized pigs.

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  • A novel paper biosensor based on [email protected]–NH2

    using a novel recombinant pseudorabies virus Hassana K. Oyibo 1,2 †,Petr Znamenskiy 1,3,Hysell V. Oviedo 1,4,Lynn W. Enquist 5 andAnthony M. Zador 1 * 1 Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA 2 Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland 3

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  • Viral Clearance in AAV Purification: Case Study ...

    2018-1-23 · 【Method】A gE/gI-deleted transferring plasmid pMD-LA-RA and a recombinant plasmid carrying EGFP gene were constructed according to the sequence of epidemic porcine pseudorabies virus. The homologous

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  • CRISPR-Cas12a-assisted nucleic acid detection | Cell

    2021-4-16 · Study Design: To assess the robustness of the affinity resin and monolith polishing step within REGENXBIO’s downstream process ( 7 ), we selected “center-point” and “worst-case” processing parameters for our viral clearance spiking experiments (Table 1). For each run, we spiked in-process AAV material with model viruses or MVM-MVPs to ...

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  • Frontiers | A NanoLuc Luciferase Reporter

    2020-9-22 · Pseudorabies (PR), also known as Aujeszky's disease, is an acute infectious disease of pigs, resulting in significant economic losses to the pig industry in many countries. Since 2011, PR outbreaks have occurred in many Bartha-K61-vaccinated pig farms in China. The emerging pseudorabies virus (PRV) variants possess higher pathogenicity in pigs and mice than the strains isolated before.

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  • Isolation of gE gene deleted pseudorabies virus by

    A simple, convenient method employing a gE-specific neutralizing monoclonal antibody (mAb; FTpn3) for isolation of the gE gene-deleted recombinant pseudorabies virus (PRV) is described. FTpn3 secreting hybridoma was obtained by fusing PRV-immunized BALB/c splenocytes with myeloma cells. To construct gE gene deleted PRV, a 5.7 kbp DNA fragment with deletion of the gE gene was engineered and cloned.

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  • Sensitive immunoassay for porcine pseudorabies

    2012-12-28 · We report on a novel immunoassay for porcine pseudorabies virus (PRV) antibody that is based on fluorescence signal amplification induced by silver(I) ion exchange in CdSe nanocrystals. An antigen-antibody-secondary antibody sandwich structure was first formed from PRV, PRV antibody, and CdSe-labeled rabbit anti-pig antibody. Then, the Cd(II) ions in the CdSe labels were released by a …

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  • ACCEPTED MANUSCRIPT OPEN ACCESS

    2021-5-19 · Pseudorabies (pseudorabies, PR) is an acute infectious disease caused by pseudorabies virus (pseudorabies virus, PRV) infection in pigs, cattle, sheep, dogs and other animals, pigs are the main host and source of infection. In January 2012, PRV was observed in pigs on several farms in northern and eastern China. In Shandong

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  • 2 simplex virus reactivation - bioRxiv.org

    2020-11-19 · method has been developed, using pseudorabies virus (PRV) (W. 74 W. Liu et al. 2008). The method centers on a microfluidic chamber system that directs the growth 75 of axons into a fluidically isolated environment and recapitulates all known aspects of neuron-to-76 cell spread. Microfluidic chip devices indeed have been rapidly improved.

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  • Identification of central nervous system neurons that ...

    1997-12-29 · Pseudorabies virus, Bartha's K strain... Identification of central nervous system neurons that innervate the bladder body, bladder base, or external urethral sphincter of female rats: A transneuronal tracing study using pseudorabies virus - Marson - 1997 - Journal of Comparative Neurology - …

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  • Design of fusion protein for efficient preparation of ...

    2021-5-9 · Cyanovirin-N (CVN) is a cyanobacterial protein with potent neutralizing activity against enveloped virus. To achieve the economic and functional production of CVN, the CVN N-terminally fused with CL7(A mutant of the Colicin E7 Dnase) was utilized to improve the solubility and stability of CVN fusion protein (CL7-CVN). Additionally, to improve the detection limit of existing PRV diagnostic ...

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  • RESEARCH Open Access Specific, simple and rapid

    2017-8-25 · using the LAMP method to determine whether they were infected by PCV2. PCV2 was detected in 95 clini-cal samples. Discussion In this study, we developed a visual and rapid detection method for PCV2 using the optimized LAMP technique. Compared to conventional PCR analysis, the LAMP method has advantages such as time-saving, low cost and ease of ...

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  • The Potency Tests of Vaccines - Creative Biolabs

    Lab Chip(影响因子: 6.7) 2013,12,1495-1499. Xueen Fang etal.Loop-mediated isothermal amplification establishment for detection of pseudorabies virus. Journal of Virological Methods 2008,151, 35–39. Xueen Fang etal.One New Method of Nucleic Acid

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  • Generation and characterization of UL41 null

    2018-8-2 · Using this method we were able to rapidly generate the UL41 null and revertant viruses of PRV variant. Compared to wild type (JS-2012), the UL41 null virus showed significantly smaller plaques ... Pseudorabies virus (PRV) is a member of the alphaher-pesviridae subfamily [1]. It is the etiologic agent of Aujeszky’s disease, which causes severe ...

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  • Frontiers | A NanoLuc Luciferase Reporter

    2020-9-22 · Pseudorabies (PR), also known as Aujeszky's disease, is an acute infectious disease of pigs, resulting in significant economic losses to the pig industry in many countries. Since 2011, PR outbreaks have occurred in many Bartha-K61-vaccinated pig farms in China. The emerging pseudorabies virus (PRV) variants possess higher pathogenicity in pigs and mice than the strains isolated before.

    Get Price
  • ACCEPTED MANUSCRIPT OPEN ACCESS

    2021-5-19 · Pseudorabies (pseudorabies, PR) is an acute infectious disease caused by pseudorabies virus (pseudorabies virus, PRV) infection in pigs, cattle, sheep, dogs and other animals, pigs are the main host and source of infection. In January 2012, PRV was observed in pigs on several farms in northern and eastern China. In Shandong

    Get Price
  • Characterization of the neuroinvasive profile of a ...

    New Method: In this report we define the neuroinvasive profile and reporter expression of a new recombinant of the Bartha strain of pseudorabies virus (PRV). The new recombinant, PRV-290, expresses the mTurquoise2 fluorophor and is designed to complement other isogenic recombinants of Bartha that express different reporters of infection.

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  • Pseudorabies Virus Expressing Enhanced Green

    with a pseudorabies virus were examined in vitro by using whole-cell patch-clamp techniques. A strain of pseudorabies virus (PRV 152) isogenic with the Bartha strain of PRV was constructed to express an enhanced green fluorescent protein (EGFP) from the human cytomeg-alovirus immediate early promoter. Unilateral PRV 152 injections into

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  • Evaluation of serological pseudorabies tests for the ...

    2018-3-3 · Pseudorabies (PR) is a viral disease of swine caused by the pseudorabies virus (PRV). Depending on the age and reproductive status of the pigs, PR is char-acterized by a range of clinical signs, including mild respiratory disease, reproductive failure, and death. 16 Losses due to PR have led to the development of vac-

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  • Retrograde labeling of neurons using pseudorabies virus

    2020-6-2 · This TechNote describes fluorescent retrograde labeling of neurons via pseudorabies viral infection of axons isolated within the axonal compartment of XonaChips®. Results using this method are illustrated using E18 rat hippocampal neurons and similar results are found using human stem cell derived neurons. Fig. 1: Retrograde labeled

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  • [PDF] c-fos Expression in Bladder-Specific Spinal

    Purpose c-fos expression in spinal neurons that are activated by lower urinary tract stimulation are not organ specific. In this experiment, we demonstrated changes of c-fos expression in bladder-specific preganglionic neurons (PGNs) and interneurons using pseudorabies virus (PRV). Materials and Methods Forty Sprague-Dawley rats were used. We identified the neuronal pathway associated with the ...

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  • Enhancing expression of the pseudorabies virus ...

    2017-7-8 · The ygEN31–270-based indirect sandwich ELISA may provide an alternative method for developing a diagnostic kit with easy manipulation and low cost. Introduction Pseudorabies virus (PRV) is an alphaherpesvirus that causes pseudorabies (also known as Aujeszky's disease) in swine, which causes severe economic losses worldwide (Mettenleiter 2000 ).

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  • A GFP-tagged version of the pseudorabies virus protein ...

    2019-6-20 · mutant was constructed by using the previous one as a template. UL56 M6 and M9 were constructed by using the forward primer UL56 GFP F and reverse primer containing the mutant site, and the termin-ation codon TGA was followed by a KpnIsite.UL56 M7, 8 and 10 were constructed by using the same method as described for the M1 construct except that

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  • Long non-coding RNA LNC_000641 regulates

    2021-3-25 · Long non-coding RNAs (lncRNAs) are a new arm of gene regulatory mechanism as discovered by sequencing techniques and follow-up functional studies. The lncRNAs regulation of pseudorabies virus (PRV) infection has rarely been reported so far. Using RNA sequencing analysis, 225 lncRNAs with significant altered expressions in 3D4/21 cells infected with PRV (ZJ01) were identified.

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  • Porcine Pseudorabies Disease Virus PRV-gE Antibodies

    2019-4-11 · Porcine Pseudorabies Disease Virus PRV-gE Antibodies ELISA Kit Catalog No: E-AD-E021 96T This manual must be read attentively and completely before using this product. If you have any problems, please contact our Technical Service Center for help. Phone: 240-252-7368(USA) 240-252-7376(USA) Email: [email protected]

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  • Research Article Construction of a gE-Deleted

    2019-7-31 · Construction of a gE-Deleted Pseudorabies Virus and Its Efficacy to the New-Emerging Variant PRV Challenge ... by detection of gE and gB protein using indirect uorescence assay (b).-week-old pigs for the e cacy study. Our results showed ... dewaxed as routine method by …

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  • Viral Clearance in AAV Purification: Case Study ...

    2021-4-16 · Study Design: To assess the robustness of the affinity resin and monolith polishing step within REGENXBIO’s downstream process ( 7 ), we selected “center-point” and “worst-case” processing parameters for our viral clearance spiking experiments (Table 1). For each run, we spiked in-process AAV material with model viruses or MVM-MVPs to ...

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  • A Method To Define the Carboxyl Terminal of Proteins ...

    2020-3-31 · 职称 教授学位 博士邮箱 [email protected] 教育经历 2004 年7月-2008年7月 荷兰内梅亨大学 , 博士1999年9月-2002年7月 四川农业大学,硕士 1995年9月-1999年7月 四川农业大学,学士 科研和工作经历 2012年12月-至今 华中农业大学动科动 ...

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  • A new immunochromatographic assay for on-site

    2019-5-25 · Accurate definition of the carboxyl terminal of proteins is necessary for elucidating posttranslational processing at the C-terminal and more generally for characterizing protein primary structures. Here, we describe a strategy for isolating and characterizing the C-terminal peptide of a protein after proteolysis with endoprotease Lys-C. Isolation is achieved using anhydrotrypsin, a ...

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  • A method for the identification of pseudorabies virus ...

    A method for the identification of pseudorabies virus protein and angiotensin AT 1A receptor mRNA expression in the same CNS neurons. ... In the present study pseudorabies virus retrogradely transported from the kidney was localised using immunohistochemistry and mRNA for the angiotensin II AT 1A receptor was detected by hybridisation ...

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  • Demonstration of pseudorabies viral antigen in thick

    Pseudorabies (Aujeszky's) virus antigens were labeled in thick and ultrathin tissue sections of young pig brain and liver tissue, using an indirect immunogold method. Antigens were tagged with 20 nm gold particles. Controls proved the specificity of the reaction in paraffin sections and ultrathin epoxy sections. Immunogold staining was compared ...

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  • Neuron-to-cell spread of pseudorabies virus in a ...

    In this paper, we describe an in vitro method for studying neuron-to-cell spread of alphaherpesviruses using a compartmented culture system. Using pseudorabies virus as a model, we infected neuron cell bodies grown in Teflon chambers and observed spread of infection to nonneuronal cells plated in a different compartment.

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  • CRISPR/Cas9-Constructed Pseudorabies Virus

    2021-2-24 · Using this method, fluorophore-tagged viruses can be produced to facilitate screening. The CRISPR/Cas9 toolbox has already been exploited for its gene editing potential in herpesviruses, including herpes simplex virus (HSV), PRV, and cytomegalovirus (CMV) ( 11 , – 14 ).

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  • Construction of bovine herpesvirus-1 (BHV-1)

    Using this method, we constructed three recombinants in which the pseudorabies virus (PRV) thymidine kinase (tk) gene was inserted at three different sites in the unique short region of BHV-1. These three sites are located in the open reading frame of gE, gG and gI genes. Previously, two sites (tk and gC) had been used to insert foreign DNA ...

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  • Transcriptome-wide survey of pseudorabies virus

    2021-6-19 · Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very complex transcriptome. Here, we present a large RNA-Seq dataset, derived from both short- and long-read sequencing. The dataset contains 1.3 million 100?bp paired-end reads that were obtained from the Illumina random-primed …

    Get Price
  • The recombinant pseudorabies virus expressing

    2020-11-16 · The eukaryotic expression vector pcDNA3.4-(N-CD2v)-His was constructed using pcDNA3.4 (purchased from Addgene, MA, USA). The extracellular region of CD2v (46–615 bp, encoding 16-205aa) synthesized by the Wuhan GeneCreate Biological Engineering (Hubei, China) was inserted into pcDNA3.4 and fused with the His-tag at the C-terminus.

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  • The recombinant pseudorabies virus expressing African ...

    2020-11-16 · the Karber method as follows: 2× 10 4 Vero cells in 90 μl DMEM were mixed with 10 μl virus solution and plated onto 96-well plates for tenfold gradient dilution, and 8 multiple wells were set for each dilution gradient. e cytopathic eect was observed every 24 h until no change appeared in any well. e titer’s calculation formula is gTCl DI 50

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  • An inactivated gE-deleted pseudorabies vaccine provides ...

    2017-8-23 · Pseudorabies, caused by pseudorabies virus(PRV), has leaded to significant economic losses to the pig industry in many countries. Pseudorabies is a porcine infectious dis-ease that can be effectively controlled using gene deletion marked vaccines and serum monitoring, enabling differen-tiation of vaccinated from infected animals [1–3]. However,

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  • Demonstration of pseudorabies viral antigen in thick

    Pseudorabies (Aujeszky's) virus antigens were labeled in thick and ultrathin tissue sections of young pig brain and liver tissue, using an indirect immunogold method. Antigens were tagged with 20 nm gold particles. Controls proved the specificity of the reaction in paraffin sections and ultrathin epoxy sections. Immunogold staining was compared ...

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  • Transcriptome-wide survey of pseudorabies virus

    2021-6-19 · Pseudorabies virus (PRV) is an alphaherpesvirus of swine. PRV has a large double-stranded DNA genome and, as the latest investigations have revealed, a very complex transcriptome. Here, we present a large RNA-Seq dataset, derived from both short- and long-read sequencing. The dataset contains 1.3 million 100?bp paired-end reads that were obtained from the Illumina random-primed …

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  • Molecular Biology of Pseudorabies Virus: Impact on ...

    SUMMARY Pseudorabies virus (PRV) is a herpesvirus of swine, a member of the Alphaherpesvirinae subfamily, and the etiological agent of Aujeszky's disease. This review describes the contributions of PRV research to herpesvirus biology, neurobiology, and viral pathogenesis by focusing on (i) the molecular biology of PRV, (ii) model systems to study PRV pathogenesis and neurovirulence, (iii) PRV ...

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  • Fluorescence-Based Monitoring of In Vivo Neural

    2009-9-9 · The study of coordinated activity in neuronal circuits has been challenging without a method to simultaneously report activity and connectivity. Here we present the first use of pseudorabies virus (PRV), which spreads through synaptically connected neurons, to express a fluorescent calcium indicator protein and monitor neuronal activity in a living animal.

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  • The recombinant pseudorabies virus expressing

    2020-11-16 · The eukaryotic expression vector pcDNA3.4-(N-CD2v)-His was constructed using pcDNA3.4 (purchased from Addgene, MA, USA). The extracellular region of CD2v (46–615 bp, encoding 16-205aa) synthesized by the Wuhan GeneCreate Biological Engineering (Hubei, China) was inserted into pcDNA3.4 and fused with the His-tag at the C-terminus.

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  • Antiviral Activity of Germacrone against Pseudorabies ...

    2019-11-26 · source of continued epidemics [9,10]. So far, the most e ective method of prevention and control of pseudorabies is vaccination. The emergence of PRV variant strains brought huge economic losses to the pig industry since classical vaccines were not …

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  • UNIVERSITI PUTRA MALAYSIA ESTABLISHMENT OF A

    2019-7-10 · BASED VIRAL ASSAY FOR PSEUDORABIES VIRUS INFECTION By PARISA HONARI November 2011 Chairman: Assoc. Prof. Zeenathul Nazariah Allaudin, PhD Faculty: Veterinary Medicine Studies were conducted to establish a viral based biochip using Surface Plasmon Resonance (SPR) technology for detection of Pseudorabies virus (PrV.

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  • CRISPR/Cas9‐mediated 2‐sgRNA cleavage facilitates ...

    2018-3-6 · This method could result in 100% knockout of ≤3500 bp of viral nonessential large fragments. Furthermore, knockin efficiency was significantly improved by using 2 sgRNAs and was also correlated with the number of background viruses. We also demonstrated that the background viruses were all 2-sgRNA-mediated knockout mutants.

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  • Simplified Iohexol-Based Method for Measurement of ...

    2021-5-23 · Currently, no simple method is available to measure kidney function in goats (with or without impaired kidney function). Therefore, we developed a simple method to measure the kidney function in goats and pigs, which is based on a single injection of iohexol and requires three blood samples. Subsequently, kidney function can be calculated using a

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