Proteomic alteration of PK-15 cells after infection by ...
2014-8-8 · Cell culture and virus infection. Porcine circovirus type 2 strain, WG09 (GenBank accession no. GQ845027), was isolated from an intensive pig farm in Shanghai, China, in 2009. The virus stock was a fourth-passage cell culture prepared in PK-15 cells with a titer of 10 6.0 TCID 50 /mL. PK-15 cells were grown in Dulbecco’s Modified Eagle’s Medium supplemented with 10 % fetal bovine serum (GIBCO, …
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