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    Overview The Invitrogen T-REx System is a tetracycline-regulated mammalian expression system that uses regulatory elements from the E. coli Tn10-encoded tetracycline (Tet) resistance operon (Hillen and Berens, 1994; Hillen et al., 1983). Tetracycline regulation in the T-REx System is based on the binding of tetracycline to the Tet repressor and derepression of the promoter controlling ...

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  • Tetracycline inducible expression—Tet-One systems

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    Thermo Fisher expicho transient expression system ExpiCHO S Cells are derived from a non engineered subclone that has been screened and isolated from CHO S Chinese hamster ovary CHO cells 1 They are a core component of the ExpiCHO Expression System Kit Cat No A29133 ExpiCHO S Cells are maintained in suspension culture have minimal tendency to clump and grow to high density in …

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    Three experiments were performed with similar results and a representative experiment is shown. (TIF) Video S2 Movie showing migration of PP1 siRNA-treated parental HeLa Tet-Off cells in a physiological EF (cathode to the right, t = 3 h). (MOV) We acknowledge the University of Kansas Specialized Chemistry Center as the supplier of the ML141 probe.

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  • A Role for PP1/NIPP1 in Steering Migration of Human

    2012-7-16 · PP1 and NIPP1 are Required for Random and Directional Migration of HeLa and PC-3-M Cells in Response to Electrical Guidance Cues. A very recent study has shown that treatment of enteric neural crest cells with okadaic acid, an inhibitor of protein phosphatases 1 and 2A, induces undirected cell protrusions and random cell movements .Hence, we investigated a potential role for PP1 in regulating ...

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  • A Role for PP1/NIPP1 in Steering Migration of Human

    Kaposi's sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus implicated in AIDS-related neoplasms. Previously, we demonstrated that the early lytic gene product K-bZIP is a transcriptional repressor that affects a subset of viral gene transcriptions mediated by the viral transactivator K-Rta (Y. Izumiya et al. J. Virol.

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    2011-7-5 · As doxycyclin is used to induce ATR-KD expression, and PARP is a key enzyme in DNA damage signalling and repair, this could significantly compromise analysis of the data. However, we found that doxycyclin (2 μg ml –1) did not inhibit PARP activity in L1210 cells or in GM847KD cells pre-incubated for 48 h with doxycyclin (Figure 1A).

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  • A role for PP1/NIPP1 in steering migration of human

    2012-7-16 · PP1 and NIPP1 are Required for Random and Directional Migration of HeLa and PC-3-M Cells in Response to Electrical Guidance Cues. A very recent study has shown that treatment of enteric neural crest cells with okadaic acid, an inhibitor of protein phosphatases 1 and 2A, induces undirected cell protrusions and random cell movements .Hence, we investigated a potential role for PP1 in regulating ...

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    KGA-2727 is a potent, selective, high-affinity inhibitor of sodium glucose cotransporter 1 (SGLT1) with Ki of 97.4 nM and 43.5 nM for human SGLT1 and rat SGLT1, respectively. The selectivity ratios (Ki for SGLT2/Ki for SGLT1) of KGA-2727 are 140 (human) and 390 (rat). KGA-2727 exhibits antidiabetic efficacy in rodent models. S8939.

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  • Testsystemen voor microbiologie | VWR

    2020-8-14 · Total RNA was extracted using the RNeasy Plus Kit (catalog no. 74134, QIAGEN) according to the manufacturer’s instructions. RNA samples were treated with Turbo DNase (catalog no. AM1907, Thermo Fisher Scientific) followed by quality assessment using the Agilent TapeStation High Sensitivity RNA Kit (catalog no. 5067-5576, Agilent).

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    2021-2-26 · A SARS-CoV-2 variant containing a D614G substitution in the spike protein shows enhanced binding to human ACE2, increased replication in human cell cultures and a …

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  • Carbazole | Sigma-Aldrich

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  • pcd inserts for turning and milling aluminum alloy ...

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    Suitable AAV vectors for expressing the dsRNA featured in the invention, methods for constructing the recombinant AV vector, and methods for delivering the vectors into target cells are described in Samulski R et al. (1987), J. Virol. 61: 3096-3101; Fisher K J et al. (1996), J. Virol, 70: 520-532; Samulski R et al. (1989), J. Virol. 63: 3822 ...

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    2018-1-10 · Background Senescence is a fundamental biological process implicated in various pathologies, including cancer. Regarding carcinogenesis, senescence signifies, at least in its initial phases, an anti-tumor response that needs to be circumvented for cancer to progress. Micro-RNAs, a subclass of regulatory, non-coding RNAs, participate in senescence regulation. At the subcellular level …

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  • (PDF) PRK1/PKN1 controls migration and metastasis

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    2018-2-21 · 2. Fisher’s systemic contact dermatitis. The term is widely used in dermatology [14]. Nevertheless, it is a misnomer, due to the lack of precise meaning in the definition itself. In essence, the most appropriate expression could be systemic reactivation of allergic contact dermatitis (SRCD) [3].

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